Oceanography: Collection, identification, classification,
testing, culturing and rearing of marine flora
and fauna. Dissolved oxygen content analysis by the
Winkler Titration Method. Use of chlorophyll rig,
phytoplankton and zooplankton nets, and various trawls.
Preservation of organisms in formalin and alcohol.
Extensive use of compound and observation microscopes.
Oceanography: Sea water analysis of the following
elements, compounds or properties and their respective
instrumentation or methods used to analyze them:
Chlorinity and Salinity - Mohr Volumetric, and Volhard
(Potentiometric) Methods and Stoichiometry.
- Inductive and YSI portable Salinometers.
Ion Concentration - Electrometric (Beckman Expandometric
- Titration with strong acid and stoichiometry using
pH and Chlorinity results.
Dioxide - Van Slyke Gas Analysis Method.
Silicate - Nessler Tubes (a Colorimetric Method).
and Reactive Nitrate - Nessler Tubes and Atomic Absorption
Spectrophotometry (Beckman Du Spectrophotometer Model
- Flame Emission Photometry (Hitachi Flame Photometer
fitted with a Phototube Multiplier No. 4300, Modified
Flame Emission Attachment No. 9220 and a Filter added
to the Monochromator System).
Deep Sea Reversing Thermometers. Submarine Photometer.
Transmismometer. NIO (National Institute of Oceanography)
and Nansen Bottles. Use of sextant, Loran, Secci Discs,
Anemometers and assorted hydrowire hardware.
Chemical analysis of substrates. Precision Depth Recorder
Analyses. Use of various Grab and Core samplers.
- Aquatic Research Institute, Port of Stockton, 15th
and F Port Roads. Mailing address P.O. Box 648, Stockton,
CA 95201. Dr. Robert R. Rofen - Director, (209) 466-5324.
Accountable for the collection (SCUBA) and classification
of marine invertebrates, especially hydroids; also
the care and rearing of the institutes fresh water
and marine life. 1966-68.
Research Member - University of California, San
Diego, Marine Life Research Group, Scripps Institution
of Oceanography, La Jolla, CA. Dissolved Oxygen Titration
Analysis. Published UC-SIO data report CalCOFI (California
Cooperative Fisheries Investigation) Reasearch Vessel
New Horizon. 1984.
Basic and Fortran IV on RSTS. Microsoft Windows, Basic
and Graphics on microprocessors.
Chemistry: Classical Quantitative Analysis - Gravimetric
Analysis of Soluble Samples of: Silver Chloride for
% Chloride; Barium Sulfate for % Sulfate. Titrimetric
Analysis of Soluble Samples of: Unknown for % Chloride,
Soda Ash for % Sodium Carbonate, Sodium Oxalate for
% Oxalate. Titrimetric Analysis of Copper in Brass
for % Copper. Volumetric Analysis of Iron Ore Sample
for % Iron. Nonclassical Quantitative Analysis - Use
of pH meters with various types of electrodes, for
example, Potentiometric Analysis of: A Chloride-Iodide
mixture, a Carbonate-Bicarbonate mixture, Iron (II)
with Cerium (IV); and Conductometric Titrations: Acid-Base,
and Precipitation. Electrogravimetric Analysis of
% Copper in a Copper Sulfate solution. Analysis of
Calcium by Atomic Absorption Spectrophotometry (a
Perkin Elmer Model 103 with a Photoemission Attachment).
Chemistry: Synthesis and Purification of Acetanilide.
Fractional Distillation and Gas Chromatography of
Cyclohexene and Toluene. Identification of unknown
Hydrocarbons. Free Radical Chlorination. Dehydration
of Methylcyclohexanol. Synthesis of Benzoic Acid by
the Gringard Reaction. Identification of Unknown Aldehydes,
Ketones and Amines. Use of Fridal Crafts Reaction
to Alkylate the Aromatic (Benzene) Ring.
Anatomy and Physiology: Measurement of Osmolality
of organisms bodily fluids by Vapor Pressure Osmometer
and/or Freezing Point Depression compared with Melting
Point. Flame Emission Photometry to find organisms
Sodium concentration. Oxygen consumption by the Winkler
Titration Method. Loading and Unloading of Oxygen
by Respiratory Pigments, i.e., Absorption Spectra
of Blood Pigments evaluated by Spectronic 20 (Bausch
& Lomb) Spectrophotometer. Measure of Myocardial Action
Potential (Contractions) by Wick Electrode, Amplifier
and Oscilloscope. Q-10 (the change in Biological Systems)
determined by Physiograph. LD 50 (Lethal Dose) and
LCT (Lethal Concentration times Time) experiments.
Use of Oxygen Meters.